Forskolin-stimulated adenylyl cyclase activity

anno   04.04.2017  



To read this story in full you will need to login or make a payment see right. View our privacy policy and use of cookies. Login Cart To read this story in full you will need to login or make a payment see right. Faculty Position for Research Director of forskolib-stimulated Animal BSL3 Facility at Duke-NUS. Send to a friend.



We use cookies to improve your experience with our site. Accept and close More info. Login Cart To activiyy this story in full you will need to login or make a payment see right. Nature6 November doi Received 28 April. Accepted 6 August Yehia Daaka 1. Lefkowitz 1 Correspondence to: Robert J. Lefkowitz 1 Correspondence and requests for materials should be addressed to R. Top of page Abstract Forskolin-stimulated adenylyl cyclase activity of the G-protein-coupled receptors for hormones that bind to the cell surface can signal to the interior of the cell through several different classes of G protein 1, 2, 3, 4.

For example, although most of the actions of the prototype 2 -adrenergic receptor are mediated through G s proteins and the cyclic-AMP-dependent protein kinase PKA system 5, 6-adrenergic receptors can also couple to G i proteins 1, 2. Here we investigate the mechanism that cyyclase the specificity of this coupling. We show that in HEK cells, stimulation of mitogen-activated protein MAP kinase by the 2 -adrenergic receptor is addenylyl by the subunits of pertussis-toxin-sensitive G proteins through a pathway involving the non-receptor tyrosine kinase c-Src and the G protein Ras.

Activation of this forskolin-stimulated adenylyl cyclase activity by the 2 -adrenergic receptor requires that the receptor be phosphorylated by PKA because it is blocked by H, an adenylul of PKA. Additionally, a mutant of the receptor, which lacks the sites normally phosphorylated by PKA, can activate adenylyl cyclase 5the enzyme that forskolin-sstimulated cAMP, but not MAP kinase.

Our results demonstrate that a mechanism previously shown to mediate uncoupling of the 2 -adrenergic receptor from G s and thus heterologous desensitization 7 PKA-mediated receptor phosphorylationalso serves to 'switch' coupling of this receptor from G s to G i and initiate a new set of signalling events. To read this story in full you will need to login or make a payment see right.

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Send to a friend. Export citation Export forskolin-stimulated adenylyl cyclase activity Rights and permissions Order commercial reprints. Search Pubmed for Yehia Daaka Louis M. Faculty Position for Research Director of the Animal BSL3 Facility at Duke-NUS. Program and Integrated MS-Ph. Program of IBS School-UST.

Institute for Basic Science. More science jobs Switching of the coupling of the 2 -adrenergic receptor to different G proteins by protein kinase A Yehia Daaka 1. Howard Hughes Medical Institute, Departments of Medicine and Biochemistry, BoxDuke University Medical Center, Durham, North CarolinaUSA Correspondence to: Robert J.



Forskolin review: What is Forskolin?





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